Additional Selective and Differential Cell Culture Media
NEOGEN MacConkey Agar, 10 kg
MacConkey Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Conforms to Harmonized USP/EP/JP Requirements and FDA/BAM. MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Escherichia coli from non-sterile products. Conforms to USP/EP/JP performance specification. Gelatin serves as source of carbon and nitrogen. Lactose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. Escherichia coli can ferment lactose to produce acid which results in a pH drop. This is indicated by neutral red resulting in pink colonies.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-agar
NEOGEN Oxford Listeria Agar, 10 kg
Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water.For more information visit https://www.neogen.com/solutions/Microbiology/oxford-listeria-agar
NEOGEN Campy Cefex Agar, 10 kg
Campy Cefex Agar is used for the selective isolation of Campylobacter spp in a laboratory setting. Campy Cefex Agar is not intended for use in the diagnosis of disease or other conditions in humans.Poultry is a primary reservoir of Campylobacter spp. and may cause contamination of more than 80% of chicken carcasses. Campy Cefex Agar is described by Stern et al. This medium was formulated to provide selective isolation of cephalothin-resistant Campylobacter species such as C. jejuni and C. coli.For more information visit https://www.neogen.com/solutions/Microbiology/campy-cefex-agar
NEOGEN Maximum Recovery Diluent (Tryptone Salt Broth), 5 kg
Maximum Recovery Diluent (Tryptone Salt Broth) is used as an isotonic diluent for maximum recovery of microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. An osmotically controlled solution which is an alternative to, and a replacement for, 1/4 strength Ringer’s Tablets (NCM0191K). The presence of a low level of peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringer’s Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent (1-2 hours). This formula is recommended by ISO 6887-1:2017 For more information visit https://www.neogen.com/solutions/Microbiology/maximum-recovery-diluent-tryptone-salt-broth
NEOGEN Modified Buffered Peptone Water with Pyruvate, 5 kg
Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-buffered-peptone-water-with-pyruvate
NEOGEN Bismuth Sulphite Agar, 10 kg
Bismuth Sulfite Agar is used for the selective isolation of Salmonella spp. In a laboratory setting. Bismuth Sulfite Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to FDA/BAM formulation. Salmonellosis can result from consumption of raw, undercooked, or improperly processed foods contaminated with Salmonella. U. S. federal guidelines require various poultry products to be routinely monitored before distribution for human consumption. Bismuth Sulfite Agar is a modification of Wilson and Blair formula. The typhoid organism grows luxuriantly on the medium, forming characteristic black colonies. Gram-positive bacteria and coliforms are inhibited on Bismuth Sulfite Agar. The inhibitory action of Bismuth Sulfite Agar permits the use of a large inoculum, increasing the possibility of recovering pathogens that may be present in small numbers.For more information visit https://www.neogen.com/solutions/Microbiology/bismuth-sulfite-agar
NEOGEN Brucella Agar, 5 kg
Brucella Agar is used for the cultivation of Brucella spp. and other fastidious microorganisms in a laboratory setting. Brucella Agar is not intended for use in the diagnosis of disease or other conditions in humans.Brucella Agar is prepared according to the APHA formula for Albimi Broth. Brucella Agar is a general-purpose medium for the cultivation of Brucella spp. and fastidious microorganisms including Streptococcus pneumoniae, Streptococcus viridans, and Neisseria meningitidis. With the addition of blood, Brucella Agar is used to determine bacterial hemolytic reactions. Brucella Agar can also be used as a base for the isolation of Campylobacter spp.For more information visit https://www.neogen.com/solutions/Microbiology/brucella-agar
NEOGEN Tetrathionate (TT) Broth Base, Hajna, 5 kg
Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-tt-broth-base-hajna
NEOGEN Dextrose Tryptone Broth, 5 kg
Dextrose Tryptone Broth is used for cultivation of mesophilic or thermophilic spoilage microorganisms from food in a laboratory setting. Dextrose Tryptone Broth is not intended for use in the diagnosis of disease or other conditions in humans.In the 1930’s, the National Canners Association specified use of Dextrose Tryptone Agar for isolating “flat sour” organisms from food products. “Flat sour” spoilage of canned foods is caused by Bacillus coagulans (Bacillus thermoacidurans). Bacterial growth results in a 0.3 – 0.5 drop in pH, while ends of the can remain flat. B. coagulans is a soil microorganism and found in canned tomato products and dairy products. Conditions favorable for multiplication of the bacterium can result in spoilage of the food product.For more information visit https://www.neogen.com/solutions/Microbiology/dextrose-tryptone-broth
NEOGEN Buffered Listeria Enrichment Broth, 10 kg
Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth
NEOGEN Orange Serum Agar, 5 kg
Orange Serum Agar is used for the cultivation of aciduric microorganisms associated with spoilage of products and is not intended for use in the diagnosis of disease or other conditions in humans.The low pH of fruit juices makes citrus fruit products susceptible to spoilage by yeasts, molds, and the bacteria Lactobacillus and Leuconostoc. In the 1950’s, Hays investigated spoilage in frozen concentrated orange juice. He found that an agar medium containing orange serum (juice) was superior to Lindegren Agar in isolating the microorganisms responsible for spoilage causing a buttermilk off-odor. Murdock, Folinazzo, and Troy found Orange Serum Agar, pH 5.4 to be a suitable medium for growing Leuconostoc, Lactobacillus, and yeasts. Orange Serum Agar is recommended for examining fruit beverages.For more information visit https://www.neogen.com/solutions/Microbiology/orange-serum-agar
NEOGEN Lauryl Sulfate Broth with MUG, 10 kg
Lauryl Sulfate Broth with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli in a laboratory setting. Lauryl Sulfate Broth with MUG is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby.For more information visit https://www.neogen.com/solutions/Microbiology/lauryl-sulfate-broth-with-mug
NEOGEN Lysine Iron Agar, 5 kg
Lysine Iron Agar is used for the differentiation of microorganisms on the basis of lysine decarboxylase and hydrogen sulfide production. Lysine Iron Agar is not intended for use in the diagnosis of disease or other conditions in humans. Lysine Iron Agar is prepared according to the formulation of Edwards and Fife, who developed the medium to detect Salmonella arizonae. S. arizonae ferments lactose rapidly, and the authors found expected H2S production on Triple Sugar Iron Agar was suppressed. Detection of S. arizonae is important because it has been implicated in food borne infections. By eliminating lactose and incorporating lysine, Edwards and Fife devised a medium differentiating enteric bacilli based on their ability to decarboxylate or deaminate lysine and produce abundant hydrogen sulfide. This medium is recommended for detecting rapid lactose-fermenting S. arizonae. For more information visit https://www.neogen.com/solutions/Microbiology/lysine-iron-agar
NEOGEN Casein Peptone Type I, 10 kg
Casein Peptone Type I is an enzymatic hydrolysate of casein for use in preparing microbiological culture media in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.Casein Peptone Type I is an enzymatic hydrolysate of casein, rich in peptones and amino acids (including tryptophane). This peptone can be utilized by most bacteria as a growth substrate.For more information visit https://www.neogen.com/solutions/Microbiology/casein-peptone-type-1
NEOGEN A-1 Medium, 5 kg
A-1 Medium is used for the detection of coliform organisms in water and food in a laboratory setting. A-1 Medium is not intended for use in the diagnosis of disease or other conditions in humans.Since the early 1900’s enumeration of coliform organisms, specifically E. coli, have been used to determine water purity. Elevated temperature and most-probable-number (MPN) methods are routinely used for analysis of water and food samples for presence of fecal coliforms. A-1 Medium was formulated to hasten E. coli recovery and reduce the incidence of false positive cultures.In 1972 Andrews and Presnell developed A-1 Medium. This medium recovers E. coli from estuarine water in 24 hours instead of 72 hours, and in greater numbers without the pre-enrichment step. A-1 Medium can be used in a single step procedure for the detection of fecal coliforms in source water, seawater, treated wastewater, and foods.For more information visit https://www.neogen.com/solutions/Microbiology/a-1-medium